Functional and molecular responses in Mytilus edulis hemocytes exposed to bacteria, Vibrio splendidus.
Identifieur interne : 000467 ( Main/Exploration ); précédent : 000466; suivant : 000468Functional and molecular responses in Mytilus edulis hemocytes exposed to bacteria, Vibrio splendidus.
Auteurs : Marion Tanguy [France] ; Patty Mckenna ; Sophie Gauthier-Clerc ; Jocelyne Pellerin ; Jean-Michel Danger ; Ahmed SiahSource :
- Developmental and comparative immunology [ 1879-0089 ] ; 2013.
English descriptors
- KwdEn :
- Animals, Cell Adhesion, Cells, Cultured, Defensins (biosynthesis), Down-Regulation, Glutathione Peroxidase (biosynthesis), Hemocytes (immunology), Hemocytes (metabolism), Muramidase (biosynthesis), Mytilus edulis (immunology), Mytilus edulis (microbiology), Phagocytosis (immunology), Proteasome Endopeptidase Complex (biosynthesis), Respiratory Burst (immunology), Superoxide Dismutase (biosynthesis), Up-Regulation, Vibrio (classification), Vibrio (immunology).
- MESH :
- chemical , biosynthesis : Defensins, Glutathione Peroxidase, Muramidase, Proteasome Endopeptidase Complex, Superoxide Dismutase.
- classification : Vibrio.
- immunology : Hemocytes, Mytilus edulis, Phagocytosis, Respiratory Burst, Vibrio.
- metabolism : Hemocytes.
- microbiology : Mytilus edulis.
- Animals, Cell Adhesion, Cells, Cultured, Down-Regulation, Up-Regulation.
Abstract
This study aims at examining the morphological, functional and molecular responses of Mytilus edulis hemocytes exposed to different strains of Gram-negative bacteria Vibrio splendidus (a virulent strain V. splendidus LGP32, V. splendidus LGP32 Δvsm without metalloprotease and an environmental type strain V. splendidus 7SHRW) at a 1:3 ratio for 2, 4, and 6 h. Our data showed that hemocytes could have a discriminative capacity towards microorganisms. Both V. splendidus LGP32 strains had an effect on hemocyte adhesion, phagocytosis abilities and oxidative burst, whereas the environmental strain 7SHRW induced weak and delayed hemocyte responses. At a molecular level, differential levels of candidate transcripts were measured in M. edulis hemocytes exposed to V. splendidus LGP32-GFP and 7SHRW. Mainly, a down-regulation of defensin was recorded in hemocytes exposed to V. splendidus LGP32. A significant up-regulation of lysozyme and proteasome 26S was observed at 2 h followed by a down-regulation at 4 and 6 h of exposure to the LGP32 strain. Similarly, SOD and GPx genes were up-regulated 2 h post-exposure to LGP32 strain and their expressions decreased after 4 and 6 h post-exposure. Further analysis is however needed in a near future to relate the transcript level variations with the physiological process.
DOI: 10.1016/j.dci.2012.10.015
PubMed: 23228459
Affiliations:
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Mckenna</name></author><author><name sortKey="Gauthier Clerc, Sophie" sort="Gauthier Clerc, Sophie" uniqKey="Gauthier Clerc S" first="Sophie" last="Gauthier-Clerc">Sophie Gauthier-Clerc</name></author><author><name sortKey="Pellerin, Jocelyne" sort="Pellerin, Jocelyne" uniqKey="Pellerin J" first="Jocelyne" last="Pellerin">Jocelyne Pellerin</name></author><author><name sortKey="Danger, Jean Michel" sort="Danger, Jean Michel" uniqKey="Danger J" first="Jean-Michel" last="Danger">Jean-Michel Danger</name></author><author><name sortKey="Siah, Ahmed" sort="Siah, Ahmed" uniqKey="Siah A" first="Ahmed" last="Siah">Ahmed Siah</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2013">2013</date><idno type="RBID">pubmed:23228459</idno><idno type="pmid">23228459</idno><idno type="doi">10.1016/j.dci.2012.10.015</idno><idno type="wicri:Area/PubMed/Corpus">000165</idno><idno type="wicri:Area/PubMed/Curation">000165</idno><idno 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Havre</wicri:regionArea><placeName><region type="region" nuts="2">Région Normandie</region><region type="old region" nuts="2">Haute-Normandie</region><settlement type="city">Le Havre</settlement></placeName></affiliation></author><author><name sortKey="Mckenna, Patty" sort="Mckenna, Patty" uniqKey="Mckenna P" first="Patty" last="Mckenna">Patty Mckenna</name></author><author><name sortKey="Gauthier Clerc, Sophie" sort="Gauthier Clerc, Sophie" uniqKey="Gauthier Clerc S" first="Sophie" last="Gauthier-Clerc">Sophie Gauthier-Clerc</name></author><author><name sortKey="Pellerin, Jocelyne" sort="Pellerin, Jocelyne" uniqKey="Pellerin J" first="Jocelyne" last="Pellerin">Jocelyne Pellerin</name></author><author><name sortKey="Danger, Jean Michel" sort="Danger, Jean Michel" uniqKey="Danger J" first="Jean-Michel" last="Danger">Jean-Michel Danger</name></author><author><name sortKey="Siah, Ahmed" sort="Siah, Ahmed" uniqKey="Siah A" first="Ahmed" last="Siah">Ahmed Siah</name></author></analytic><series><title level="j">Developmental and comparative immunology</title><idno type="eISSN">1879-0089</idno><imprint><date when="2013" type="published">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Cell Adhesion</term><term>Cells, Cultured</term><term>Defensins (biosynthesis)</term><term>Down-Regulation</term><term>Glutathione Peroxidase (biosynthesis)</term><term>Hemocytes (immunology)</term><term>Hemocytes (metabolism)</term><term>Muramidase (biosynthesis)</term><term>Mytilus edulis (immunology)</term><term>Mytilus edulis (microbiology)</term><term>Phagocytosis (immunology)</term><term>Proteasome Endopeptidase Complex (biosynthesis)</term><term>Respiratory Burst (immunology)</term><term>Superoxide Dismutase (biosynthesis)</term><term>Up-Regulation</term><term>Vibrio (classification)</term><term>Vibrio (immunology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Defensins</term><term>Glutathione Peroxidase</term><term>Muramidase</term><term>Proteasome Endopeptidase Complex</term><term>Superoxide Dismutase</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Vibrio</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Hemocytes</term><term>Mytilus edulis</term><term>Phagocytosis</term><term>Respiratory Burst</term><term>Vibrio</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Hemocytes</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Mytilus edulis</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Adhesion</term><term>Cells, Cultured</term><term>Down-Regulation</term><term>Up-Regulation</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">This study aims at examining the morphological, functional and molecular responses of Mytilus edulis hemocytes exposed to different strains of Gram-negative bacteria Vibrio splendidus (a virulent strain V. splendidus LGP32, V. splendidus LGP32 Δvsm without metalloprotease and an environmental type strain V. splendidus 7SHRW) at a 1:3 ratio for 2, 4, and 6 h. Our data showed that hemocytes could have a discriminative capacity towards microorganisms. Both V. splendidus LGP32 strains had an effect on hemocyte adhesion, phagocytosis abilities and oxidative burst, whereas the environmental strain 7SHRW induced weak and delayed hemocyte responses. At a molecular level, differential levels of candidate transcripts were measured in M. edulis hemocytes exposed to V. splendidus LGP32-GFP and 7SHRW. Mainly, a down-regulation of defensin was recorded in hemocytes exposed to V. splendidus LGP32. A significant up-regulation of lysozyme and proteasome 26S was observed at 2 h followed by a down-regulation at 4 and 6 h of exposure to the LGP32 strain. Similarly, SOD and GPx genes were up-regulated 2 h post-exposure to LGP32 strain and their expressions decreased after 4 and 6 h post-exposure. Further analysis is however needed in a near future to relate the transcript level variations with the physiological process.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Haute-Normandie</li><li>Région Normandie</li></region><settlement><li>Le Havre</li></settlement></list><tree><noCountry><name sortKey="Danger, Jean Michel" sort="Danger, Jean Michel" uniqKey="Danger J" first="Jean-Michel" last="Danger">Jean-Michel Danger</name><name sortKey="Gauthier Clerc, Sophie" sort="Gauthier Clerc, Sophie" uniqKey="Gauthier Clerc S" first="Sophie" last="Gauthier-Clerc">Sophie Gauthier-Clerc</name><name sortKey="Mckenna, Patty" sort="Mckenna, Patty" uniqKey="Mckenna P" first="Patty" last="Mckenna">Patty Mckenna</name><name sortKey="Pellerin, Jocelyne" sort="Pellerin, Jocelyne" uniqKey="Pellerin J" first="Jocelyne" last="Pellerin">Jocelyne Pellerin</name><name sortKey="Siah, Ahmed" sort="Siah, Ahmed" uniqKey="Siah A" first="Ahmed" last="Siah">Ahmed Siah</name></noCountry><country name="France"><region name="Région Normandie"><name sortKey="Tanguy, Marion" sort="Tanguy, Marion" uniqKey="Tanguy M" first="Marion" last="Tanguy">Marion Tanguy</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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